This page provides specifications of knee specimen OKS003. This is a young, healthy, female specimen.
Specimen Characteristics
Left knee
Gender: Female
Age: 25 years
Race: White
Height: 1.73 m
Weight: 68 kg
BMI: 22.8
Serologically tested: Yes.
X-ray:
ImageLink(oks003.png, width=600, alt=Experimentation Workflow)
Experimentation
-- ["aerdemir"] DateTime(2014-11-30T23:52:27Z) All, please complete relevant sections of this page during and after experimentation.
Specimen
Reference Specification: ["Specifications/Specimens"] - [http://wiki.simtk.org/openknee/Specifications/Specimens?action=recall&rev=40 Revision as of November 30, 2014]
Leading Team Member: Snehal Chokhandre
Supporting Team Members: Ahmet Erdemir
Timeline: November 30, 2014 @ ~ 9:00 AM (specimen pulled out of freezer)
Data Location: In this page.
Protocol Deviations:
- None.
Notes:
- None.
Specimen Preparation
Reference Specification: ["Specifications/SpecimenPreparation"] - [http://wiki.simtk.org/openknee/Specifications/SpecimenPreparation?action=recall&rev=139 Revision as of November 30, 2014]
Leading Team Member: Tara Bonner
Supporting Team Members: Snehal Chokhandre, Craig Bennetts, Ahmet Erdemir
Timeline:
- Thawing: ~9:00 AM November 30, 2014 - ~9:00 AM December 1, 2014
- Dissection: ~9:15 AM - ~10:15 AM December 1, 2014
- Assembly of Optotrak Base Plugs: ~10:15 AM - ~11:30 AM December 1, 2014
- Assembly of Registration Markers: ~11:30 AM - ~12:15 PM December 1, 2014
- Probing of Anatomical Landmarks: ~1:15 PM - ~3:00 PM December 1, 2014
- Probing of Registration Markers: ~1:15 PM - ~3:00 PM December 1, 2014
Data Location:
Protocol Deviations:
- Femur registration markers were assembled medially, laterally, and posteriorly (rather than anteriorly or anteromedially). Registration marker probing order was also changed to lateral, medial, posterior. Tibia registration markers were assembled per the specification. Probing order is the same as the specification: lateral medial posterior.
- Ten points (not twelve) were collected on each registration marker. The specifications may need to change as per Robb this has been how the system was set up all the time.
Notes:
- Dissected and keeping skin-fat-muscle layers from thigh and shank.
- Patella Optotrak marker needed to be replaced. Probing of patella registration marker was repeated for this reason.
Joint Imaging
Specimen Preparation
Reference Specification: ["Specifications/SpecimenPreparation"] - [http://wiki.simtk.org/openknee/Specifications/SpecimenPreparation?action=recall&rev=139 Revision as of November 30, 2014]
Leading Team Member: Craig Bennetts
Supporting Team Members: Snehal Chokhandre
Timeline: ~3:00 PM - 3:30 PM December 1, 2014 (patella registration marker will be placed next day)
Data Location:
Protocol Deviations:
- None.
Notes:
- None.
Imaging
Reference Specification: ["Specifications/ExperimentationAnatomicalImaging"] - [http://wiki.simtk.org/openknee/Specifications/ExperimentationAnatomicalImaging?action=recall&rev=120 Revision as of December 1, 2014]
Leading Team Member: Craig Bennetts
Supporting Team Members: Snehal Chokhandre, Ahmet Erdemir
Timeline:
- Specimen Placement in Transportation Fixture: 8:30 AM - 9:00 AM December 2, 2014
- Imaging: ~9:45 AM - ~11:30 AM December 2, 2014
Data Location:
Data was received from the imaging facilities and uploaded to in-house data management server (http://cobicore.lerner.ccf.org/midas; only accessible within Cleveland Clinic network). Raw DICOM files were stored in the folder Open Knee(s) --> Private --> oks003 --> MRI --> DICOM
DICOM files were converted to NIfTI files, stored in the folder Open Knee(s) --> Private --> oks003 --> MRI --> NIFTI of the in-house data management server. NIfTI files were also disseminated to the public in the 'Downloads' section of the project website, https://simtk.org/home/openknee, under the package oks003.
Data storage and dissemination were accomplished based on the updated imaging specifications, see [http://wiki.simtk.org/openknee/Specifications/ExperimentationAnatomicalImaging?action=recall&rev=118 Revision as of April 16, 2015].
Protocol Deviations:
- None.
Notes:
- To be able to identify where the patella is after the specimen is in the opaque bag, a double sided tape was secured to connect the transportation fixture to the bag. This helps orienting the specimen better in the MRI.
- General purpose imaging attachment:general-purpose.png
- Cartilage imaging attachment:cartilage.png
- Connective tissue imaging attachment:connective-tissue.png
Joint Mechanics
Equipment Preparation
Reference Specification: ["Specifications/PressureCalibration"] - [http://wiki.simtk.org/openknee/Specifications/PressureCalibration?action=recall&rev=17 Revision as of December 2, 2014]
Leading Team Member: Robb Colbrunn
Supporting Team Members: Ahmet Erdemir, Nicole Nassif
Timeline: ~1:30 PM - ~2:00 PM December 2, 2014
Data Location:
Protocol Deviations:
- None.
Notes:
- Sensor model is 5051, label is 5051-P1-16813DT1-1200.
- A single pass equilibration at 6 bars was conducted.
- A double pass equilibration was conducted at an additional pressure level of 3 bars, by using single pass 6 bars data. Software reordered the passes.
Specimen Preparation
Reference Specification: ["Specifications/SpecimenPreparation"] - [http://wiki.simtk.org/openknee/Specifications/SpecimenPreparation?action=recall&rev=139 Revision as of November 30, 2014]
Leading Team Member: Tara Bonner
Supporting Team Members:
Timeline:
- Specimen preparation for TFJ testing: ~1:15 PM - ~2:00 PM December 2, 2014
- Specimen preparation for PFJ testing: ~7:00 AM - ~8:30 AM December 3, 2014
Data Location:
Protocol Deviations:
Notes:
Testing
Reference Specification: ["Specifications/ExperimentationJointMechanics"] - [http://wiki.simtk.org/openknee/Specifications/ExperimentationJointMechanics?action=recall&rev=94 Revision as of December 3, 2014]
Leading Team Member: Tara Bonner
Supporting Team Members: Robb Colbrunn
Timeline:
- Specimen Mounting for TFJ testing: 1:00 PM December 3, 2014
- Testing of TFJ: 2:30 PM - 5:00 PM December 3, 2014
- Specimen Mounting for PFJ testing: 8:30 AM December 3, 2014
- Testing of PFJ: 12:30 PM - 2:30 PM December 3, 2014
Data Location:
Protocol Deviations:
Notes:
- In tibiofemoral joint testing, passive flexion (for coordinate system optimization) was conducted at 50 N compressive load. Specifications state a 100 N compressive load should be used. Nonetheless, as noted by Tara, all previous specimens has been tested under 50 N compressive load for coordinate system optimization. The relevant protocol may need to be updated this.
- In tibiofemoral joint testing, robotic testing of combined loading at 90 degrees flexion ended prematurely. An additional trial was conducted to complete the tests.
In patellofemoral joint testing, a coordinate system transformation matrix in SimVitro (the matrix that establishes the relationship between the tibia and load cell) needed to be mirrored to accommodate the left knee.
- In patellofemoral joint testing, at 0 degrees flexion multiple trials were collected for 20, 100, 200, 300, 400, 500 N quadriceps loading. This was necessitated by troubleshooting tendon freeze clamping and tendon actuator response.
- Version of simVITRO software used was unreleased revision 3024 of the repository. The files can be found in svn://dev.lerner.ccf.org/data1/svn/repos/colbrunn-svn/Projects/Numbered Projects/2013CB-031 Open Knee/Original simVITRO-Tested on oks001 oks002 oks003 oks004
Tissue Mechanics
Specimen Preparation
Reference Specification:
Leading Team Member: Snehal Chokhandre
Supporting Team Members: Craig Bennetts
Timeline:
- Soft tissue dissection: ~3:00 PM - ~5:00 PM December 3, 2014
- Cartilage dissection: ~9:30 AM - 12:00 PM December 4, 2014
Data Location:
Protocol Deviations:
- Due to delay in patellofemoral joint testing, tissue dissection was not completed on the same day; all ligament, tendon, menisci segments were dissected and frozen. Bone and cartilage were kept in the fridge another night for dissection in the following morning.
Notes:
- Various secondary tissues were dissected and stored: semitendinosus, patellofemoral ligament (medial and lateral), lateral tibiofemoral capsule, fat bursa, transverse ligament.
Testing
Reference Specification:
Leading Team Member:
Supporting Team Members:
Timeline:
Data Location:
Protocol Deviations:
Notes:
Data Analysis
Pictured below are the Anterior Posterior laxity graph, the IR/ER laxity graph, and the Varus/Valgus laxity graph.
attachment:oks006_2LaxityGraphs.png