Target Outcome
Acquisition of cadaver knee specimens of desired type
Target Specimens
Knee from a young, healthy, male donor. Donor age < 35 years.
Knee from a young, healthy, female donor. Donor age < 35 years.
Knee from an elderly, healthy, male donor. Donor age > 65 years.
Knee from an elderly, healthy, female donor. Donor age > 65 years.
Knee from an elderly, osteoarthritic, male donor. Donor age > 65 years; moderate to severe based on Kellgren-Lawrence grading (1,2).
Knee from an elderly, osteoarthritic, female donor. Donor age > 65 years; moderate to severe based on Kellgren-Lawrence grading (1,2).
Specimens should meet the following criteria:
- Entire leg from hip to ankle.
- Age: 18 years old - 80 years old
- Height: 1.50 m - 1.90 m
- Weight: 45 kg - 90 kg
- BMI: 18.5 - 24.9 (normal range)
- Serologically tested.
Specimens will be excluded when
- Knee injury, surgeries, or inflammatory arthritis.
Secondary Specimens
Besides the primary specimens described above, more specimens can be tested in future to include conditions such as obesity, and different gender and age combinations. These will be listed in this section.
Specimen Acquisition
Science Care Inc. (Phoenix, AZ), http://www.sciencecare.com/
National Disease Research Interchange (Philadelphia, PA), http://ndriresource.org/
Deliveries should be to:
- Snehal Chokhandre
- Lerner Research Institute, 2111 E. 96th St. Cleveland, OH. 44195
-- ["aerdemir"] DateTime(2013-10-22T15:08:47Z) Add delivery address to receive the specimens
Specimen Evaluation
- Before testing, the specimens will be evaluated by an orthopaedic surgeon for their suitability to their assigned group. When possible, pre-purchase radiographic information will be may utilized for this purpose.
Specimen Numbering Convention
- Specimen will be numbered irrespective of their type as they are acquired and tested, e.g., 001, 002.
Tissue Samples from Specimens
- Primary samples: ACL, PCL, MCL, LCL, Menisci, medial and lateral femoral and tibial cartilage, patellar cartilage, cartilage in femoral groove,capsule, patellar ligament, quadriceps tendon, MPFL. At least on sample from each tissue will be required for mechanical testing and following constitutive modeling.
- Secondary samples: When possible, additional tissue samples will be prepared along with the primary samples and frozen for later use. This may include different tissue types or more sampling of the same tissue from different regions.
References
Berger MJ, Kean CO, Goela A, Doherty TJ. Disease severity and knee extensor force in knee osteoarthritis: Data from the Osteoarthritis Initiative. Arthritis Care Res (Hoboken). 2012 May;64(5):729-34.
Braun HJ, Gold GE. Diagnosis of osteoarthritis: imaging. Bone. 2012 Aug;51(2):278-88.
-- ["aerdemir"] DateTime(2013-10-22T15:01:07Z) Please provide links to PubMed along with these references