TableOfContents

Target Outcome

Prerequisites

Infrastructure

For more details see ["Infrastructure/ExperimentationMechanics"].

Previous Protocols

For more details see ["Specifications/Specimens"].

For more details see ["Specifications/ExperimentationAnatomicalImaging"]

For more details see ["Specifications/Registration"].

For more details see ["Specifications/ExperimentationJointMechanics"].

Protocols

Joint Testing Preparation

Initial Specimen Preparation

Anchor(Marker_Mounting)

Optoelectronic Marker Mounting

attachment:optotrak_mounting_assembly.png

Anchor(MRI_markers_prep)

MRI Registration Marker Mounting

Tibia and Femur

attachment:knee_with_registration_markers.png

attachment:spherical_marker.png Anchor(Patella_MRI_marker)

Patella

attachment:patella_registration.png

Specimen Digitization/Registration Procedure

MRI Compatible Markers

  1. Patella
    • The digitization of points and the registration of the [:Specifications/Registration#Patella_MRI_CS:patella MRI marker coordinate system] can be done prior to specimen preparation.
    • The plug of the optoelectronic marker [:Specifications/SpecimenPreparation#Marker_Mounting:fixture] should be rigidly secured to a piece of wood that is fixed in space.
    • The patella optoelectronic marker should be secured to the plug and the position/orientation data should be recorded to identify where the sensor is in space
    • The [:Specifications/SpecimenPreparation#Patella_MRI_marker:patella MRI registration marker] attachment should be mounted on the plug and the 6 divots should be digitized using the digitizing probe.

attachment:digitizing_probe.png

Anatomical Landmarks

After the [:Specifications/SpecimenPreparation#Marker_Mounting:optoelectronic markers] have been secured to the specimen, the anatomical landmarks can be digitized with respect to the markers.

A digitizing probe should be used to take each point with respect to the bone's respective marker.

  1. The [:Specifications/Registration#Tibia_CS:tibia coordinate system] requires the digitization of the following points
    • T1. Medial tibial plateau
    • T2. Lateral tibial plateau
    • T3. Medial malleolus of the tibia
    • T4. Lateral malleolus of the fibula
  2. The [:Specifications/Registration#Femur_CS:femur coordinate system] requires the digitization of the following points
    • F1. Lateral epicondyle of the femur
    • F2. Medial epicondyle of the femur
    • F3-F6. 4 points around the epiphyseal line of the femur
  3. The [:Specifications/Registration#Patella_CS:patella coordinate system] requires the digitization of the following points
    • P1. Most lateral point
    • P2. Most medial point
    • P3. Most superior point
    • P4. Most inferior point

Tissue testing sample preparation

Dissection

Tissue sample punches

Vibratome

Sample labelling

Storage