Target Outcome
Material behavior for all primary and secondary tissues necessary for required representative constitutive models.
Prerequisites
Infrastructure
For more details see ["Infrastructure/ExperimentationMechanics"].
Previous Protocols
For more details see ["Specifications/Specimens"].
For more details see ["Specifications/ExperimentationAnatomicalImaging"]
For more details see ["Specifications/Registration"].
For more details see ["Specifications/SpecimenPreparation"].
For more details see ["Specifications/ExperimentationJointMechanics"]
Protocols
Ligaments
Conditions
- Uniaxial tensile test under stress relaxation condition at 5% and 10% strain at 20%/s strain rate.
Measurements
- Force-deformation behaviour
Operating Procedure
- sample dimensions
- testing condition
- Fixtures, bath, filter etc
- preload, preconditioning, stress relaxation (relaxation duration etc)
-- ["snehalkc"] DateTime(2013-10-28T20:57:10Z) Images and links to specific sections in specimen preparation and infrastructure needed.
Cartilage
Conditions
- Uniaxial tension
- Preload: 0.1 N,
- Stress relaxation: 5%, 10% strain at 20%/s (Hold time after each strain application will be 60 min)
Measurements
- Force-deformation behaviour
Operating Procedure
- Dumbbell shaped, 10mm by 2mm test length samples will be prepared for all ligaments.
- Samples will be harvested from the mid-substance region.
- Sample thickness will be measured before each test using a constant-pressure linear variable displacement transducer (LVDT) probe.
- Sand paper and tissue adhesive will be used along with the metal clamps to prevent the test samples from slipping during the mechanical tests.
- Tests will be conducted on a Instron 5543 series testing system (Instron, Canton, Massachusetts) using a 50N load cell.
- Specimens will be kept immersed in a saline bath and kept at 37 °C during testing.
- Stress relaxation tests will be conducted on each sample.
Menisci
Conditions
- Confined compression and uniaxial tensile tests under stress relaxation condition at 5%,10% and 15% strain at 20%/s strain rate.
Measurements
- Force-deformation behaviour
Operating Procedure
- sample dimensions
- testing condition
- Fixtures, bath, filter etc
- preload (both tests), preconditioning, stress relaxation (relaxation duration etc)
Tendons
Conditions
Measurements
Operating Procedure
Capsule
Conditions
Measurements
Operating Procedure
References
1. Seitz, Andreas Martin, Fabio Galbusera, Carina Krais, Anita Ignatius, and Lutz Dürselen. “Stress-relaxation Response of Human Menisci Under Confined Compression Conditions.” Journal of the Mechanical Behavior of Biomedical Materials 26 (October 2013): 68–80. doi:10.1016/j.jmbbm.2013.05.027. http://www.sciencedirect.com/science/article/pii/S175161611300204X
-- ["aerdemir"] DateTime(2013-10-15T13:42:41Z) I provided an outline above to help categorize activities.
-- ["aerdemir"] DateTime(2013-10-15T13:42:41Z) Should sample descriptions go to "Specifications/Specimens"? Ideally tissue-specific operating procedure should be followed for any given sample from that tissue.
Notes
- Number of samples:
- Sample locations:
- Sample dimensions:
- Sample preparations:
- Testing equipment:
- Dimension measurement:
- Testing protocol:
- Confined compression
- Uniaxial tensile test